Composite

Part:BBa_K2992043:Design

Designed by: Yaseen Tengur   Group: iGEM19_Nottingham   (2019-10-01)


FAST reporter construct with Pfdx 5-UTR+RBS.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 369


Design Notes

FAST is originally derived from Halorhodospira halophile but codon optimised for Clostridium spp. [Street et al, 2019 DOI: 10.1128/AEM.00622-19].


Source

C. pasteurianum and C. sporogenes DNA. FAST is originally derived from Halorhodospira halophile but codon optimised for Clostridium spp. [Street et al, 2019 DOI: 10.1128/AEM.00622-19].

References