Composite
Part:BBa_K2992043:Design
Designed by: Yaseen Tengur Group: iGEM19_Nottingham (2019-10-01)
FAST reporter construct with Pfdx 5-UTR+RBS.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 369
Design Notes
FAST is originally derived from Halorhodospira halophile but codon optimised for Clostridium spp. [Street et al, 2019 DOI: 10.1128/AEM.00622-19].
Source
C. pasteurianum and C. sporogenes DNA. FAST is originally derived from Halorhodospira halophile but codon optimised for Clostridium spp. [Street et al, 2019 DOI: 10.1128/AEM.00622-19].